[Histonet] Immunoperoxidase with DAB (Single Labeling) and Histofluorescence (Double labeling) Protocol

[Fred Ross-Cisneros]

Dear All,
 
Could someone be so kind to share or advise me on a  protocol for both
immunoperoxidase using DAB (single labeling) and immunofluorescence
(using double labels) on formalin-fixed paraffin-embedded (FFPE) human
brain and optic nerve tissues? I need to identify myelin basic protein
(MBP), glial fibrillary acidic protein (GFAP), neurofilaments (NF), and
ED-1 for macrophages. I will do the immunofluorescence on a confocal
microscope. 
 
Thank you.
 
Fred    
 

Fred N. Ross-Cisneros

Neuro-Ophthalmology Lab

USC Keck School of Medicine and

Doheny Eye Institute

1355 San Pablo Street, DVRC 311

Los Angeles, CA 90033-1026

Tel.: (323) 442-6667

Fax: (323) 442-6688