[Histonet] frozen tissues

[Till, Renee]

Has anyone done regular paraffin embedded IHC with frozen tissues? I am
going to need to do PCNA, Tunel, and an Agouti antibody on frozen mouse
livers. I have only tried this once before years ago with rat prostate
and testis, and those still worked fine. I wasn't sure whether the type
of tissue would matter, or the stain being done. They wanted me to check
before we start, though I will probably test just a couple first. I
could do frozens...but paraffin would be so much easier for me.

 

Renee' Till