[Histonet] Cutting frozen fat
abright <@t> brightinstruments.com
Tue Mar 15 03:30:13 CST 2005
You should have no problem sectioning mesenteric and epididymal fat at a specimen temperature of -30ºC, but if your knife and anti-roll plate are at a warmer temperature than the specimen this would explain the results you report.
Please come back to me if you require further assistance.
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From: pam plumlee [mailto:paw555 <@t> yahoo.com]
Sent: 15 March 2005 03:13
To: HistoNet Server
Subject: [Histonet] Cutting frozen fat
Hi all: I have a project that the researcher wants
frozen sections on mouse fat for immunofluorescence.
I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam
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