[Histonet] Cutting frozen fat

Alan Bright abright <@t> brightinstruments.com
Tue Mar 15 03:30:13 CST 2005


You should have no problem sectioning mesenteric and epididymal fat at a specimen temperature of -30ºC, but if your knife and anti-roll plate are at a warmer temperature than the specimen this would explain the results you report.

Please come back to me if you require further assistance.
Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
PE29 6EU

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com

-----Original Message-----
From: pam plumlee [mailto:paw555 <@t> yahoo.com] 
Sent: 15 March 2005 03:13
To: HistoNet Server
Subject: [Histonet] Cutting frozen fat

Hi all:    I have a project that the researcher wants
frozen sections on mouse fat for immunofluorescence. 
I froze mesenteric and epididymal fat at necropsy in isopentane.  I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block.  I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help.  Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions.  Pam

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