[Histonet] Commonly used stains for kidney disease progession??
katri <@t> cogeco.ca
Wed Mar 9 20:27:08 CST 2005
When we receive a native kidney biopsy (human), the routine is to cut serial
sections at 3um and pick sections up on several slides. 3 or 4 get an H&E, 1
PAS, 1 Jones methenamine silver for basement membranes, 1 trichrome and 1
elastin stain ( we use Miller). Also one piece of the biopsy is snap frozen
for consequent IF stains for immune complexes, and one piece is fixed for
There are many different kidney diseases and combination of all these stains
will confirm or rule out many of them, but that is the pathologists' area
of expertise. I know that for instance PAS and silver stains demonstrate the
basement menbranes within the glomeruli and can indicate immune complex
deposits within them anf if so the IF will determine what kind of immune
complexes they are. These are just the routine stains, more are done if the
pathologist requests. I think your pathologist is the best source of
information in this area.
Hope this helps a bit...
Hamilton, Ontario, Canada
----- Original Message -----
From: "- -" <emerald_lake77 <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, March 09, 2005 4:17 PM
Subject: [Histonet] Commonly used stains for kidney disease progession??
> Hi everyone,
> Quick question:
> What are the commonly used stains/immunochemistry procedures to evaluate
> progression of disease within kidneys? (I may need to know WHY also in
> case I am not familiar with a certain technique, stain, or antibody)
> So far I have been told a number ranging from PAS, trichrome and a silver
> technique - but I started to become confused. They all seem great but I
> think I may need the WHY along with the WHAT when answers are being sent
> back in reply.
> As usual, thank you for your assistance.
> G.T. Hebert
> Cambridge Massachusetts
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