[Histonet] Mast cells staining with TOL

[Amira Fitieh]

Hi all,

 

We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution.

Best regards,

Amira

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Amira Fitieh
Research student
Arvid Carlsson Institute for Neuroscience
Göteborg University
Medicinaregata 11
Box 432
405 30 Göteborg
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