[Histonet] mouse heart morphology

[David McClister]

Hello,

I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles.  This lab has tried dentamold but I found it very difficult to section.  Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue?
Thanks 
David McClister, HTL (ASCP)
Medical University of South Carolina
Dept of Cardiothoracic Research
117 Doughty St
Charleston, SC 29425