[Histonet] TH and golgi-cox?

[ninian H]

Hi all,
Does anyone know about combining golgi-cox  with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and  TH positive fibers. I'll appreciate any information  that might help me to see both pyramydal cells and TH containing fibers in cortical areas. 
Also  co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion? 
Thank you in advance,
Ninian, ph.D student


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Today's Topics:

1. RE: removal of carbohydrates/glycosylation
(Edmondson David (RBV) NHS Christie Tr)


----------------------------------------------------------------------

Message: 1
Date: Wed, 2 Mar 2005 17:48:49 -0000
From: "Edmondson David \(RBV\) NHS Christie Tr"

Subject: RE: [Histonet] removal of carbohydrates/glycosylation
To: "Sharon Cooperman" 
Cc: "Histonet \(E-mail 2\)" 
Message-ID:

Content-Type: text/plain; charset="iso-8859-1"


I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof.
Any suggestions/

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sharon
Cooperman
Sent: 02 March 2005 15:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] removal of carbohydrates/glycosylation


Hi,

Does anyone know of a method to remove carbohydrate 
modifications/glycosylation from proteins in tissue sections on 
slides prior to immunohistochemistry? (I know it's a weird 
question.) Any suggestions, references, etc. would be greatly 
appreciated.

Thanks,
Sharon
-- 
Sharon Cooperman 
NIH, NICHD, CBMB 301.435-8417
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892

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End of Histonet Digest, Vol 16, Issue 5
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