[Histonet] Immunostaining on bone marrow smears

[Richard Cartun]

I've noticed a loss of immunoreactivity for certain proteins in
air-dried smears after 2-3 days.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "M.Vici" <manuvici <@t> med.unibo.it> 03/02/05 10:20AM >>>
Hi everybody!
I've just joined the histonet.
My question:
I need to immunostain old-air dried- bone marrow smears.
I'm using an anti-nucleophosmin monoclonal antibody (nucleolus).
Which fixation and peroxidase suppression protocols would you 
suggest? 

Thanks!
Manuela


 



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