[Histonet] disinfection of cryostat

[María Teresa Domínguez]

   I  usualy  use a gauze soaked with a bleach solution (5%) to clean the
   cryostat,but I turned it off first, and take it off the microtome unit
   to  clean it outside and change the knife. All this process takes half
   an our or less. Then you can turn the cryostat on to be used again.

   HT. MARIA TERESA DOMINGUEZ

   ANATOMIC PATOLOGY SERVICE

   REG. HOSPITAL OF RIO GRANDE,

   TIERRA DEL FUEGO, ARGENTINA.
   >From:     "Michael     Hetzer"     <HETZER <@t> surgery.wisc.edu>     >To:
   <histonet <@t> lists.utsouthwestern.edu>  >Subject: [Histonet] disinfection
   of cryostat >Date: Tue, 01 Mar 2005 15:22:56 -0600 > >Use 100% ETOH to
   disinfect  your  cryostat  without turning it off. We use >the last of
   the dehydrating ETOH from the previous day's staining >setup.Wear your
   PPE  and  use  4x4 gauze that is soaked with the ETOH to >the point of
   dripping a little bit. Dispose of your blade first or if >your using a
   knife  first  disifect both sides of the kife using only >upward wipes
   and  then  set  the  knife  in a safe out of the way place >inside the
   cryostat preferrably a spot that has already been cleaned. >Remove any
   waste   materials   with  the  wet  gauze  and  then  wipe  the  inner
   >surfaces.Wipe  out  any  excess ETOH with dry 4x4 gauze. It will take
   you >five minutes at the most. > >Mike Hetzer >Dermatologic Procedures
   >Madison,    WI   >   >_______________________________________________
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   >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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References

   1. http://g.msn.com/8HMAEN/2728??PS=47575