[Histonet] Hematoxylin[Scanned]

[Kemlo Rogerson]

Good girl....

Gone are the days when I had 5 litre flasks of Harris's haematoxylin
ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it
was plagiarised 'Gills' haematoxylin ripening, then some infernal lead
haematoxylin concoction that turned everything it touched black; I strangely
put on weight too.

The ripening of haematoxylin to haematin then to oxyhaematin was a delight
to witness, the latter meant you have gone too far. The ability to carry out
an H&E progressively without having to remove the cytoplasmic 'overstaining'
a delight.

I'm one for keeping 'home made' haematoxylin together with retaining the 'a'
in haematin, but on both points alas I feel I shall fail.

  

-----Original Message-----
From: Bauer, Karen [mailto:Bauer.Karen <@t> mayo.edu] 
Sent: 01 March 2005 18:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Hematoxylin[Scanned]

Hello,

I was just wondering what everyone is using for Hematoxylin these days.
We are one of those rare Histology Labs that makes their own hematoxylin
and eosin and I just got the OK from our Pathologist to try out some
commercial brands.  What do you like?  What are you using?  Good and bad
comments are greatly appreciated.

Thanks!!

Karen Bauer HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire, WI
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