[Histonet] Re: Flying brains

[Gayle Callis]

Although breathing gently into a cryostat chamber may help solve the 
problem, this is also an aerosol although more gentle than a spray 
-  neither is ideal when working with potential biohazards.  Obviously warm 
moist air is solving the problem.  We experience the "flying section 
syndrome"  in our lack of humidity climate, and it is worse when using 
colder sectioning temperatures.

Maybe a warm water dampened gauze sitting inside the chamber, or moisten 
back of gloves.  Dryer sheets to wipe down metal parts, antiroll 
plates,  knife holders, and gloves.    We cut rodent brain at -16C.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)