[Histonet] fixation question

ddittus787 <@t> aol.com ddittus787 <@t> aol.com
Wed Jun 29 14:19:24 CDT 2005

Once tissue has been properly fixed ,NBF should have no huge effect on H&E, sometimes over-fixing (2 or more fixatives) can affect special stains, IHC,IN-situ. If I were a student and had 3 fixatives I would wait to get past the formalin step to be able to purely judge fixation issues of each independent fixative. You know what we learn and what happens in the real world is always different.
-----Original Message-----
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, 29 Jun 2005 11:22:41 -0700
Subject: Re: [Histonet] fixation question

If she is comparing the effects of three different fixatives, then she should go into the post-fixative part of the schedule. Otherwise she is comparing double fixation of two of them. That is another, seperate, question. 
Bryan Llewellyn 
----- Original Message ----- From: "Scholz, Stephen J." <Stephen.J.Scholz <@t> osfhealthcare.org> 
To: <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, June 29, 2005 10:59 AM 
Subject: [Histonet] fixation question 
> Hello all; 
> I have a question about some fixatives. We have a student whom is using > our lab in conjunction with an on-line course to prepare for the exam. > She was asked to fix tissue in three different fixatives and compare the > results on H&E. She has chosen Bouins, formalin/alcohol and 10% BNF. We > use the BNF as routine in our lab. My question is after she has fixed the > tissue in the other two fixatives can she put the specimen back into the > 10% BNF for routine processing or does she need to wait until the > processor goes passed its formalin changes in order to add her specimens? > I have had no experience with using a fixative other than 10% BNF as a > primary so I don't know what to tell her. 
> Thank you for your help, 
> Steve Scholz 
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