[Histonet] fixation question

Bryan Llewellyn llewllew <@t> shaw.ca
Wed Jun 29 13:22:41 CDT 2005

If she is comparing the effects of three different fixatives, then she 
should go into the post-fixative part of the schedule.  Otherwise she is 
comparing double fixation of two of them.  That is another, seperate, 

Bryan Llewellyn

----- Original Message ----- 
From: "Scholz, Stephen J." <Stephen.J.Scholz <@t> osfhealthcare.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 29, 2005 10:59 AM
Subject: [Histonet] fixation question

> Hello all;
> I have a question about some fixatives.  We have a student whom is using 
> our lab in conjunction with an on-line course to prepare for the exam. 
> She was asked to fix tissue in three different fixatives and compare the 
> results on H&E.  She has chosen Bouins, formalin/alcohol and 10% BNF.  We 
> use the BNF as routine in our lab.  My question is after she has fixed the 
> tissue in the other two fixatives can she put the specimen back into the 
> 10% BNF for routine processing or does she need to wait until the 
> processor goes passed its formalin changes in order to add her specimens? 
> I have had no experience with using a fixative other than 10% BNF as a 
> primary so I don't know what to tell her.
> Thank you for your help,
> Steve Scholz


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