[Histonet] preservation of the stratum corneum in skin cryosections

Birthe Schnegelsberg schnegelsberg <@t> xgene.com
Wed Jun 29 10:53:30 CDT 2005


Does anybody have a method to keep the stratum corneum attached to the
epithelium using in vitro skin cryosections?

I am freezing in vitro skin without fixation in isopentane chilled in liquid
nitrogen. I don' t see any stratum corneum in my 5-7um HandE stained

Thanks, Birthe

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