[Histonet] detachment of tissue from OCT compound

Monfils, Paul PMonfils <@t> Lifespan.org
Wed Jun 29 10:35:51 CDT 2005

Someone has sent me 60 rat brains frozen in OCT compound (whole brain except
for the cerebellum and the anterior 1 cm). He wants multiple 30-micron
sections from each brain. They appear to be well infiltrated with sucrose,
and they cut smoothly on the cryostat at -20C. However, the sections of
tissue fall out of the surrounding OCT, which makes it very difficult to
pick them up on slides without a lot of wrinkles and bubbles.

Any tricks to deal with this situation?

Paul M.

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