[Histonet] Silly question about deparaffinization
pmarcum <@t> vet.upenn.edu
Wed Jun 29 09:21:59 CDT 2005
Depending on the thickness of the sections my times can vary. Sections of 4
to 6 micron are deparaffinized in our laboratory at 2 changes at 5 minutes
each with alcohols at 3 minutes each per station to water. Sections
thicker than 6 microns may go as long 15 minutes X 2 in xylenes and 5 to 10
minutes in each alcohol. I am very paranoid about getting all of the
paraffin (or MMA where I use one hour per change for xylenes) so I may go
over board. I also change my reagents more often in humid weather. I do
not use an automated stainer so agitation only takes place at the beginning
of the changes to assure the last reagent is rinsed off.
Hope this helps.
At 09:56 AM 6/29/2005, Eva C Andersson wrote:
>I am probably asking a very silly question but I recently spoke to someone
>about deparaffinization and hydration. I found that the times that they
>use and the times that we use are very different. So my question to you is
>this: What lenghts of time do you use for Xylenes, 100% alcohol, 95%
>alcohol and 70% alcohol?
>It is about deparaffinising charged (+) slides. So nothing fancy.
>Just curious. Maybe I can cut back some on the timed that we use which are:
>30min Xylenes, 10min 100%, 10min 95% and 6min 70%.
>Thanks for your responses.
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>Histonet <@t> lists.utsouthwestern.edu
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu
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