[Histonet] RE: Histonet Digest, Vol 19, Issue 39 - CD 138

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Wed Jun 29 02:39:34 CDT 2005


We have tried both the Vector clone 5F7 and the Lab Vision clone B-B4. On
our Ventana Benchmark XT, the B-B4 worked really well, but it didn't like
Vector's and only occasional cells stained.
Jacqui Malam
Royal Infirmary 
Lancaster
England

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu] 
Sent: 27 June 2005 18:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 19, Issue 39

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Today's Topics:

   1. Freezing Antobodies ! (Cesar Francisco Romero)
   2. RE: S100 staining (Malam Jacqueline)
   3. OT:: [Histonet] immunostainers (louise renton)
   4. RE: pos control for copper (Malam Jacqueline)
   5. prognostic markers and IHC staining (C. Loukas)
   6. Re: Freezing Antobodies ! (Katri Tuomala)
   7. RE: : [Histonet] immunostainers (O'Brien, Sue)
   8. S/P Pro-Texx* Mounting Medium (Atoska S. Gentry)
   9. Re: prognostic markers and IHC staining (Richard Cartun)
  10. MUM1/IRF4 and CD138 (Pat Zeitlow)
  11. RE: S/P Pro-Texx* Mounting Medium (Tom McNemar)


----------------------------------------------------------------------

Message: 1
Date: Mon, 27 Jun 2005 02:03:14 -0300
From: "Cesar Francisco Romero" <chesarato <@t> hotmail.com>
Subject: [Histonet] Freezing Antobodies !
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY10-F2891273CB5EAAB060070B5BBEE0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"; format="flowed"


   Dear People from Histonet

   I  would  like  to  know if you freeze your antibodies in Kitchen like
   Freezers ( - 18 or - 20 º C ).

   I can not afford a - 80 º C Freezer.

   Thank You in advance

   Cesar Romero

   Buenos Aires

   Argentina
     _________________________________________________________________

   Nuevo MSN Messenger [1]Una forma rápida y divertida de enviar mensajes

References

   1. http://g.msn.com/8HMAESAR/2734??PS=47575


------------------------------

Message: 2
Date: Mon, 27 Jun 2005 08:44:38 +0100
From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
Subject: [Histonet] RE: S100 staining
To: "Histonet submissions (histonet <@t> lists.utsouthwestern.edu)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B75B29D97DE3E84ABC2276497D44C33B0815FF3C <@t> rlixch>
Content-Type: text/plain

Hi Laura
We have a Benchmark XT and it works fine for us - can you let me know your
ab source, protocol and dilution - see if I can shed some light.

Jacqui Malam
Lancaster
England



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------------------------------

Message: 3
Date: Mon, 27 Jun 2005 09:51:30 +0200
From: louise renton <louise.renton <@t> gmail.com>
Subject: OT:: [Histonet] immunostainers
To: CrochiereSteve <@t> aol.com, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <e483362e05062700517959c10b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

On 6/26/05, CrochiereSteve <@t> aol.com <CrochiereSteve <@t> aol.com> wrote:
> I wholeheartedly recommend the Nemesis Immunostainer from Biocare Medical.
> I have been using this instrument for almost a year and have had no 
> technical problems with this machine.

Does anyone think about the meaning of words when they name these machines?

NEMISIS: A source of harm or ruin: 
Retributive justice in its execution or outcome:  
An opponent that cannot be beaten or overcome. 
One that inflicts retribution or vengeance. 
Nemesis Greek Mythology. The goddess of retributive justice or vengeance.



------------------------------

Message: 4
Date: Mon, 27 Jun 2005 10:30:50 +0100
From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
Subject: [Histonet] RE: pos control for copper
To: "Histonet submissions (histonet <@t> lists.utsouthwestern.edu)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B75B29D97DE3E84ABC2276497D44C33B0815FF3D <@t> rlixch>
Content-Type: text/plain

Many thanks to everyone who responded. I am now the proud possessor of a
positive control block - my world is complete !

Jacqui Malam
Lancaster
England



DISCLAIMER: This e-mail is confidential and privileged. If you are not the
intended recipient please accept our apologies; please do not disclose, copy
or distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful. Please
inform postmaster <@t> rli.mbht.nhs.uk that this message has gone astray before
deleting it.  Comments or opinions expressed in this email are those of
their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.




------------------------------

Message: 5
Date: Mon, 27 Jun 2005 12:08:00 +0000
From: "C. Loukas" <cloukas <@t> hotmail.com>
Subject: [Histonet] prognostic markers and IHC staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY102-F12F6EEA3C9EEAE95D3A77DA3EE0 <@t> phx.gbl>
Content-Type: text/plain; format=flowed

Hello all,

Although I'm new in this list, it seems that is viewed by loads of
Histologists, so I would be grateful if  someone could recommend me a couple
of review articles/textbook focusing on the assessment of the various
prognostic markers currently in use (e.g.  ER in breast cancer, MIB-1 in
oesophageal cancers), through immunohistochemical staining of tumor tissue
sections.

In particular I'm after a list of: established prognostic markers (or
stains) vs tumor type, if such a think exists.

Thanks in advance!

cl





------------------------------

Message: 6
Date: Mon, 27 Jun 2005 09:01:26 -0400
From: "Katri Tuomala" <katri <@t> cogeco.ca>
Subject: Re: [Histonet] Freezing Antobodies !
To: "Cesar Francisco Romero" <chesarato <@t> hotmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001001c57b18$5471c1a0$6a9a9618 <@t> Katri>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=response

Cesar,
If your freezer is a self defrosting kind, it is not a good idea to keep
antibodies or frozen tissue in it. There is too much temperature fluctuation
as it defrosts itself. Chest freezers keep the temperature more stable and
may be acceptable. Keep a thermometer in it to record the temperature daily.

Katri

Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message -----
From: "Cesar Francisco Romero" <chesarato <@t> hotmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 27, 2005 1:03 AM
Subject: [Histonet] Freezing Antobodies !


>
>   Dear People from Histonet
>
>   I  would  like  to  know if you freeze your antibodies in Kitchen like
>   Freezers ( - 18 or - 20 º C ).
>
>   I can not afford a - 80 º C Freezer.
>
>   Thank You in advance
>
>   Cesar Romero
>
>   Buenos Aires
>
>   Argentina
>     _________________________________________________________________
>
>   Nuevo MSN Messenger [1]Una forma rápida y divertida de enviar mensajes
>
> References
>
>   1. http://g.msn.com/8HMAESAR/2734??PS=47575
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 7
Date: Mon, 27 Jun 2005 09:38:47 -0400
From: "O'Brien, Sue" <sobrien <@t> bthosp.com>
Subject: RE: : [Histonet] immunostainers
To: "louise renton" <louise.renton <@t> gmail.com>,
	<CrochiereSteve <@t> aol.com>,	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4D95C24EC0E4C84787A6919F1165B25E24B16A <@t> btmhems01.BTHOSP.INT>
Content-Type: text/plain;	charset="us-ascii"

Steve, Sounds like the company is focusing on the "An opponent that
cannot be beaten or overcome" part of the definition. I also have a
Nemesis (since Feb) and have been VERY pleased with its performance. I
would highly recommend it to anyone considering an automated
Immunostainer.
Susan O'Brien, Histology Supervisor
Burdette Tomlin Memorial Hospital
Cape May court House, NJ

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
renton
Sent: Monday, June 27, 2005 3:52 AM
To: CrochiereSteve <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Subject: OT:: [Histonet] immunostainers

On 6/26/05, CrochiereSteve <@t> aol.com <CrochiereSteve <@t> aol.com> wrote:
> I wholeheartedly recommend the Nemesis Immunostainer from Biocare
Medical.
> I have been using this instrument for almost a year and have had no
technical
> problems with this machine.

Does anyone think about the meaning of words when they name these
machines?

NEMISIS: A source of harm or ruin: 
Retributive justice in its execution or outcome:  
An opponent that cannot be beaten or overcome. 
One that inflicts retribution or vengeance. 
Nemesis Greek Mythology. The goddess of retributive justice or
vengeance.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 8
Date: Mon, 27 Jun 2005 08:55:20 -0500
From: "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] S/P Pro-Texx* Mounting Medium
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
	<6.0.1.1.0.20050627084308.01b1a090 <@t> mailhost.vetmed.auburn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

  Good morning All, do any of you have a source for S/P Pro-Texx* Mounting 
Medium, Cat. M7635-1?  If so will you please share it with me ASAP? If it's 
no longer on the market will someone please provide ordering info for a 
comparable mounting medium? Your prompt reply will be much appreciated.
Atoska




------------------------------

Message: 9
Date: Mon, 27 Jun 2005 10:58:13 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] prognostic markers and IHC staining
To: <cloukas <@t> hotmail.com>,<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2bfdbdf.051 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

A good article dealing with breast cancer is, "Prognostic and Predictive
Factors in Breast Cancer by Immunohistochemical Analysis" by D. Craig Allred
et al. in Modern Pathology 1998:11(2):155-168.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "C. Loukas" <cloukas <@t> hotmail.com> 06/27/05 08:08AM >>>
Hello all,

Although I'm new in this list, it seems that is viewed by loads of 
Histologists, so I would be grateful if  someone could recommend me a couple

of review articles/textbook focusing on the assessment of the various 
prognostic markers currently in use (e.g.  ER in breast cancer, MIB-1 in 
oesophageal cancers), through immunohistochemical staining of tumor tissue 
sections.

In particular I'm after a list of: established prognostic markers (or 
stains) vs tumor type, if such a think exists.

Thanks in advance!

cl



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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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------------------------------

Message: 10
Date: Mon, 27 Jun 2005 10:29:39 -0500
From: "Pat Zeitlow" <pzeitlow <@t> bbpllab.com>
Subject: [Histonet] MUM1/IRF4 and CD138
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <813FB33DA405334F947F8BFC6EBD0B2A47D652 <@t> bbplsrv1.bbpl>
Content-Type: text/plain;	charset="US-ASCII"

Is anyone using MUM1/IRF4 and/or CD138 that would be willing to share
vendors, clones or protocols for use with routine FFPE IHC?
 
Pat Z
Department of Molecular Pathology
Boyce and Bynum Pathology Labs, P.C.
 


------------------------------

Message: 11
Date: Mon, 27 Jun 2005 12:00:24 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] S/P Pro-Texx* Mounting Medium
To: "'Atoska S. Gentry'" <gentras <@t> vetmed.auburn.edu>, Histonet
	<histonet <@t> pathology.swmed.edu>
Message-ID: <6CD94D97ED7D924BA5C2B588FA9528213967D5 <@t> mail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

We used Pro-tex for many years and got from Scientific Products. Two maybe
three years ago they (SP) substituted Cytoseal 60 (made by Richard Alan).
Works just as well so that's what we are using to this day.

Pro-tex is no longer available throgh Scientific Products.  The order numbe
for Cytoseal 60  M7630-2A and the phone number for Scientific Products is
(800) 964-5227.

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio 43055



-----Original Message-----
From: Atoska S. Gentry [mailto:gentras <@t> vetmed.auburn.edu] 
Sent: Monday, June 27, 2005 9:55 AM
To: Histonet
Subject: [Histonet] S/P Pro-Texx* Mounting Medium


  Good morning All, do any of you have a source for S/P Pro-Texx* Mounting 
Medium, Cat. M7635-1?  If so will you please share it with me ASAP? If it's 
no longer on the market will someone please provide ordering info for a 
comparable mounting medium? Your prompt reply will be much appreciated.
Atoska


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 19, Issue 39
****************************************


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their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.






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