[Histonet] OIL RED O - Mounting Media
mpaula <@t> rpa.fmrp.usp.br
Tue Jun 28 12:09:19 CDT 2005
I mounted my fat slide with a mounting medium specific for Oil Red O
The Protocol is:
Apathy's Mouting Media
50 g sucrose PA
50 g Acacia (gum arabic) PA
150 ml Distilled Water
0.1 g Thymol
Mix Acacia and distilled water in a Becker. Place the Becker in a pan of
boiling water until the Acacia is dissolved. Than mix sucrose until dissolved and
put in a Thymol. Refrigerate to remove air bubbles.
Carefful with bubbles.
MariaPaula Montiani Scandar
Department of Pathology
Labory of Neuropathology
School of Medicine of Ribeirão Preto
University of São Paulo
----- Original Message -----
From: "Anita Jennings" <ajennings <@t> unmc.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 22, 2005 10:42 AM
Subject: [Histonet] Oil Red O mountant
> I had the same problem. initially with my fat stain.....Poly Scientific
> K043 contains the glycerine jelly mounting medium specific for Oil Red
> coverslipping, havent had a problem since I started ordering this kit
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> No virus found in this incoming message.
> Checked by AVG Anti-Virus.
> Version: 7.0.323 / Virus Database: 267.7.11/26 - Release Date: 22/06/05
More information about the Histonet