[Histonet] RE: dyeing specific tissues for processing

krat18 <@t> aol.com krat18 <@t> aol.com
Wed Jun 22 11:22:09 CDT 2005

Don't think I've seen this mentioned yet, but our pathologists just drop hematoxylin on the tiny biopsies when they're grossing, put them on sponges or wrap in paper and drop them into the formalin.  No rinsing.  Works great.
Karen_Raterman <@t> ssmhc.com
krat18 <@t> aol.com
-----Original Message-----
From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
To: Histonet submissions (histonet <@t> lists.utsouthwestern.edu) <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, 22 Jun 2005 08:53:50 +0100
Subject: [Histonet] RE: dyeing specific tissues for processing

A method we use when processing tiny biopsies is to wrap them in a small
piece of "speci-wrap" tissue like a parcel, then place parcel into a biopsy
basket to hold it, place a couple of drops of 1% methylene blue onto the
parcel and leave for a few seconds. Remove the parcel and, holding onto it
carefully, shwish (a technical term) in a beaker of water for a few secs to
wash. Place back in the basket, put lid on, place in cassette etc and
process. The dye is enough to lightly stain the tissue so when you unwrap
it, you can see it to embed. 

Jacqui Malam

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