[Histonet] RE: dyeing specific tissues for processing

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Wed Jun 22 02:53:50 CDT 2005

A method we use when processing tiny biopsies is to wrap them in a small
piece of "speci-wrap" tissue like a parcel, then place parcel into a biopsy
basket to hold it, place a couple of drops of 1% methylene blue onto the
parcel and leave for a few seconds. Remove the parcel and, holding onto it
carefully, shwish (a technical term) in a beaker of water for a few secs to
wash. Place back in the basket, put lid on, place in cassette etc and
process. The dye is enough to lightly stain the tissue so when you unwrap
it, you can see it to embed. 

Jacqui Malam

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