[Histonet] Oil Red O

[Andrea Grantham]

I know that this stain has been discussed many times on histonet but I have 
a question that may not have been asked before:
The tissue I am working with is mouse liver from mice who are fed diets 
very high in fat or alcohol. This makes for very, very fatty livers. So 
much so that when stained with Oil Red O that the sections just ooze red. 
If we look at the slides and do quantitative analysis and/or photographs 
right away there is little or no problem but if the investigator waits a 
few days before looking at the slides they are a mess. Control slides are 
OK because they contain a normal amount of fatty tissue.
We have also found that doing the hematoxylin counterstain reduces the 
amount of Oil Red Staining so we do a set counterstained and one without 
for quantitative analysis.
Is there a way to extend the life of this stain when they can't be viewed 
immediately?
Thanks,
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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