[Histonet] Schiff's reagent in microwave?

Bonner, Janet Janet.Bonner <@t> FLHOSP.ORG
Thu Jun 16 10:53:56 CDT 2005

We did this microwaving of the Schiff reagent for awhile, but the step is
only 15 minutes at room temperature so it wasn't worth using the microwave
for this application.  The Schiff Reagent last longer, too!!   -Janet

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Jacquie.Farnsworth <@t> cls.ab.ca
Sent: Thursday, June 16, 2005 10:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Schiff's reagent in microwave?

Hi Histonetters,
Does anyone have some thoughts on microwaving Schiff's reagent?  We
currently microwave Schiff's in our plastics laboratory (for PAS stain on
our renal biopsies).  We take the heated reagent out of the microwave, and
place it in a fumehood immediately and remove slides.  One of our techs is
still having problems with the fumes.  R95 masks are available, but I am
wondering if anyone has any thoughts? Suggestions?  Does anyone else
microwave Schiff's reagent?

Thank you in advance,

Jacqueline Farnsworth
Anatomic Pathology Tech III
Calgary Laboratory Services
Jacquie.Farnsworth <@t> CLS.ab.ca
PH: (403) 944-1578
Pager: 212-8223 #1933

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