[Histonet] RE: IVIEW DAB detection kits

[White, Lori]

Hi Tracey,
I am assuming from your email address that you are from Canada?  I have been experiencing significant background staining and variability for the last couple of weeks.  I have been working with the Ventana tech support and discovered there are other "unhappy" accounts in Canada related to a specific master lot.  We seemed to have resolved the problem after receiving some new kits with a different lot #.  I am still cautious however and advise you to follow up with your Ventana rep.
Good luck!
Lori


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Sent: Saturday, June 11, 2005 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 19, Issue 17

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Today's Topics:

   1. antigen retrieval in bone paraffin sections (pex)
   2. RE: antigen retrieval in bone paraffin sections
      (Elizabeth Chlipala)
   3. IVIEW DAB detection kits (Tracey.Lenek <@t> CLS.ab.ca)


----------------------------------------------------------------------

Message: 1
Date: Sat, 11 Jun 2005 05:25:05 +0800 (CST)
From: pex <pex0220 <@t> yahoo.com.cn>
Subject: [Histonet] antigen retrieval in bone paraffin sections
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050610212505.64592.qmail <@t> web15502.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Hello, all, 

Do you have some good suggestions about antigen retrieval in bone paraffin sections? 

Thank you! 

 
Guofeng

__________________________________________________
¸Ï¿ì×¢²áÑÅ»¢³¬´óÈÝÁ¿Ãâ·ÑÓÊÏä?
http://cn.mail.yahoo.com

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Message: 2
Date: Fri, 10 Jun 2005 15:42:01 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] antigen retrieval in bone paraffin sections
To: "'pex'" <pex0220 <@t> yahoo.com.cn>,
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000201c56e05$3c16dbb0$a7d48a80 <@t> AMY>
Content-Type: text/plain;	charset="gb2312"

Guofeng

I frequently use the ready to use proteinase K from DakoCytomation on
formalin fixed, formic acid decalcified bone sections.  I get good
results using proteinase K with antibodies such as F4/80 in mouse
joints, ED-1 in rat joints.  IL-6 in both mouse and rat joints, mouse
neutrophils, factor H, BrdU and Factor VIII.  The other retrieval method
I use is BioGenex's DeCal.  I use that for CD45R in mice.  I try to stay
away from HEIR.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pex
Sent: Friday, June 10, 2005 2:25 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval in bone paraffin sections

Hello, all, 

Do you have some good suggestions about antigen retrieval in bone
paraffin sections? 

Thank you! 

 
Guofeng

__________________________________________________
¸Ï¿ì×¢²áÑÅ»¢³¬´óÈÝÁ¿Ãâ·ÑÓÊÏä?
http://cn.mail.yahoo.com
_______________________________________________
Histonet mailing list
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------------------------------

Message: 3
Date: Fri, 10 Jun 2005 16:36:12 -0600
From: <Tracey.Lenek <@t> CLS.ab.ca>
Subject: [Histonet] IVIEW DAB detection kits
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4BC300747AF87A48BCDF8E48BC2885CE010E0F10 <@t> mail1.calgary.com>
Content-Type: text/plain;	charset="iso-8859-1"

Hi,
We are a high volume IHC department and use Ventana's systems exclusively.  Over the past 6 months we have noticed inconsistent staining and batch to batch variation with different lot #'s of detection kits. Very difficult to maintain consistency with a large number of primary antibodies that we offer.  Has anyone else experienced a similar problem?
Thanks
Tracey

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