AW: [Histonet] MACCHIAVELLO STAIN

[Gudrun Lang]

I have learnd that acid solution inhibits the staining of methylenblue. We
use it after decolorisation the Ziehl-Neelson with 3% HCl-alcohol and wash
the slides very well. 

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Secholsb <@t> aol.com
Gesendet: Mittwoch, 08. Juni 2005 02:34
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] MACCHIAVELLO STAIN

Has anybody done a macchiavello stain for chlamydia?  If so,  HELP....
I am unable to get my methylene blue to stain my background.   I can leave 
the slides in methylene blue over night and it still will not  stain.  What 
could I possibly     
 be doing incorrectly?  Thanks
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet