Gudrun Lang gu.lang <@t>
Wed Jun 8 10:30:05 CDT 2005

I have learnd that acid solution inhibits the staining of methylenblue. We
use it after decolorisation the Ziehl-Neelson with 3% HCl-alcohol and wash
the slides very well. 

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von
Secholsb <@t>
Gesendet: Mittwoch, 08. Juni 2005 02:34
An: histonet <@t>
Betreff: [Histonet] MACCHIAVELLO STAIN

Has anybody done a macchiavello stain for chlamydia?  If so,  HELP....
I am unable to get my methylene blue to stain my background.   I can leave 
the slides in methylene blue over night and it still will not  stain.  What 
could I possibly     
 be doing incorrectly?  Thanks
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