[Histonet] Processing with mesh cassettes

[pmarcum <@t> vet.upenn.edu]

Be very careful adding detergent to your fixative or dipping cassettes in it. 
The result can dissolve the lipids in the cell walls and cause very serious
problems with the microscopic examination.  The cytoplasm can become very
indistinct and difficult to understand in context of the tissue.

The tissue processor manufacturers don't usually like detergent in the systems
as it can cause foaming through out the plumbing with air pockets.  

Pam Marcum

Quoting Fred Underwood <funderwood <@t> mcohio.org>:

> Hi Michelle,
> 
> I've not attempted this.  Perhaps if you exposed the cassettes to a
> solution to reduce the surface tension of the screens.  Possibly putting
> a small amount of a detergent in the first formalin.  Or dipping the
> cassettes in a detergent solution before placing them on the processor.
> 
> Fred
> 
> >>> "Michelle Becker" <mbecker <@t> pathlabinc.com> 06/07/05 10:12AM >>>
> We recently started using mesh cassettes for small biopsies, also
> called
> micromesh, histoscreen or micro biopsy cassettes, depending on the
> manufacturer. The techs, as well the pathologists, love them.
> Unfortunately, it has caused reagent carryover in our tissue processors
> (VIP
> 3000 & Leica TP1050)after only one run.  The first reagent level
> (formalin)decreases significantly; the 2nd reagent level(Prefer), as
> well
> the next 3 alcohol levels, have significant increases. The tissue
> processor
> used does not seem to make a difference. We run a 4 hour short program
> with
> vacuum for biopsies. Has anyone else had this problem and if so, how
> did you
> resolve it?  I really hate to stop using something the techs and
> pathologists love and actually agree on!
> Thank-you.
> 
> Michele Becker, HTL(ASCP)
> Histology Manager
> Laboratory Corporation of America
> Portsmouth, NH
> 
> 
> 
> 
> 
> 
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