[Histonet] Excessive background staining with Millers elastic stain

[Lachlan Smith]

Dear Subscribers

I am staining cartilage using Millers elastic fibre stain, and while elastic
fibres are staining well, there is a lot of blue background staining which
makes thresholding for histoquantitation almost impossible. My sections are
30
micron paraffin embedded. I am also oxidising with 0.5% aqueous potassium
permanaganate and bleaching with 2% oxalic acid.

Any suggestings for reducing this background staining would be greatly
appreciated.

Kind regards,

Lachlan Smith
Institute of Medical and Veterinary Science
Adelaide, Australia