[Histonet] Problems of processing brain for paraffin embeddin g

[Favara, Cynthia (NIH/NIAID)]

I do mouse and hamster and would be happy to help. If you will send me your
processing schedule, reagents used, I will take a look and see if I can pick
up on anything.
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: azita parvaneh tafreshi [mailto:aptafreshi <@t> yahoo.com] 
Sent: Saturday, July 30, 2005 1:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Problems of processing brain for paraffin embedding

Dear Members,
I have problems with paraffin processing of mouse and hamster brains and
spinal cords. I have perfused the animals with PFA 4% and I postfixed them
in the same buffer for 7-8 days and then stored in Alc 70% (up to now).
After sectioning, tissues wrinkle in a way that it is torn after flattenning
(on a slide warmer), therefore the quality is not good at all.
 
Can anyone give me hints to overcome the problem. A detailted protocol of
paraffin processing would be great.
 
Regard
A.P. Tafreshi

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