[Histonet] Problems of processing brain for paraffin embeddin
Favara, Cynthia (NIH/NIAID)
cfavara <@t> niaid.nih.gov
Sun Jul 31 06:52:21 CDT 2005
I do mouse and hamster and would be happy to help. If you will send me your
processing schedule, reagents used, I will take a look and see if I can pick
up on anything.
903 South 4th Street
Hamilton, MT 59840
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives
From: azita parvaneh tafreshi [mailto:aptafreshi <@t> yahoo.com]
Sent: Saturday, July 30, 2005 1:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Problems of processing brain for paraffin embedding
I have problems with paraffin processing of mouse and hamster brains and
spinal cords. I have perfused the animals with PFA 4% and I postfixed them
in the same buffer for 7-8 days and then stored in Alc 70% (up to now).
After sectioning, tissues wrinkle in a way that it is torn after flattenning
(on a slide warmer), therefore the quality is not good at all.
Can anyone give me hints to overcome the problem. A detailted protocol of
paraffin processing would be great.
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet