[Histonet] endogen biotin in prostata?

anh2006 <@t> med.cornell.edu anh2006 <@t> med.cornell.edu
Wed Jul 27 23:16:05 CDT 2005

You can just add your strep-HRP or ABC step to a section (without primary
or secondary) and if you get signal it is surely from the biotin
endogenous to the tissue. It is a good control to run in any given

Biotin is tricky as it often appears in very "specific" patterns and is
often misinterpreted as real staining.

I also agree HIER can dramatically increase biotin signal.

> ----- Original Message -----
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> To: "Histonetliste (Histonetliste)" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, July 27, 2005 3:58 PM
> Subject: [Histonet] endogen biotin in prostata?
>> Hi,
>> Since a few weeks our 34betaE12 shows granular spots in the cytoplasma
>> of
>> some cells in the prostata-slide. They look really sprinkled, but not in
>> all. Could that be endogen biotin?
>> Or appears biotin more homogen? Please help an unknowing histotech.
>> Gudrun Lang

More information about the Histonet mailing list