[Histonet] endogen biotin in prostata?

Katri Tuomala katri <@t> cogeco.ca
Wed Jul 27 22:40:22 CDT 2005

Do you use HIER to antigen retrieve? I have seen this type of endogenous 
biotin in heat retrieved tissue. Do a biotin block and see, if it's gone.

Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message ----- 
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
To: "Histonetliste (Histonetliste)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, July 27, 2005 3:58 PM
Subject: [Histonet] endogen biotin in prostata?

> Hi,
> Since a few weeks our 34betaE12 shows granular spots in the cytoplasma of
> some cells in the prostata-slide. They look really sprinkled, but not in
> all. Could that be endogen biotin?
> Or appears biotin more homogen? Please help an unknowing histotech.
> Gudrun Lang
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