[Histonet] Optimising the vasopressin V1b receptor stain
lilliangarrett <@t> gmail.com
Wed Jul 27 05:39:35 CDT 2005
I'm just wondering whether anyone has had success using the
vasopressin V1b receptor antibody from Alpha Diagnostics International
in brain tissue... I am having trouble optimising this stain and get
faint to no signal......
Could anyone suggest possible ways of getting the most from this staining.....
The brain tissue is fixed in 4% paraformaldehyde.... I want to stain
cryosectioned material using DAB as the cromogen.... So far I have
increased the primary antibody incubation time to 48 hours and have
heated the sections to no avail.....
Any help with this problem would be greatly appreciated...
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