[Histonet] Endogenous peroxidase blocking

Pablo Sánchez Quinteiro psanquin <@t> lugo.usc.es
Fri Jul 22 13:14:53 CDT 2005

Dear histonetters,

Thanks a lot for all your tips!

One further question. When doing a control immunohistochemical procedure
omitting the primary antibody I guess that if I have not blocked the
endogenous peroxidase activity properly I should get staining. Isnt'it? If
in case I do not get any stain may I understand that the endogenous
activity is not a real problem?

I am working with free floating sections, polyclonal antibody, ABC, DAB.

Thanks once and again 


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