[Histonet] Cytology Immuno's

[rgrow <@t> bmnet.com]

In our lab, we are making icc controls to mirror conditions for same type
of cytology specimen.  Normal tonsil, appendix, and skin (from a breast
reduction) are good controls for many IHC's.  We just call surgery and ask
them to send us some fresh when we see it on the schedule.  After gross
dictation and sectioning, we take positive charged slides and make touch
preps, smears, and swish a section in a thin prep bottle.  After slides
have dried, I package a few at a time in transport tubes, seal with
parafilm and place in a freezer.  The thin prep bottle is refrigerated. We
document the case number it was collected from, then validate.  When we
have an ICC on something we don't have cyto controls on, we use standard
FFPE controls.  So far, it has worked great.
Any comments?

We also use a daily stain validation sheet with these catagories:
acceptable, unacceptable, comments, signature, action taken, and tech.  One
side is for routine and special stains, flip over and the other side is for
IHC's.  We take to the pathologist in charge for that week with the first
slides of the day.

Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax



Message: 10
Date: Tue, 19 Jul 2005 14:38:26 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Cytology Immuno's
To: "Jean Gillson" <jean.gillson <@t> elht.nhs.uk>,
             <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPEELOCKAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain;            charset="iso-8859-1"

Jean,
we use paraffin controls for any cytology specimen. I wish we could keep
some cyto prep controls available, but we just don't have the room, nor the
specimens.
             During my CAP inspection in January, it wasn't an issue, 50
other items
were an issue, but not using FFPE controls with cytology


Joe

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jean
Gillson
Sent: Tuesday, July 19, 2005 9:41 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cytology Immuno's


Can I ask you all, what do you use as controls when doing ICC on cytology
cytospin preps or smears?  Since there isn't much sample, do you use
paraffin sections as positive controls and is that adequate control
measures?  We currently only use paraffin cytoblocks but considering ICC on
thin layer cytology preps and wondering what control material to use.
Thanks for your help.