[Histonet] Problem with Eosin Y staining

Katri Tuomala katri <@t> cogeco.ca
Tue Jul 19 12:32:15 CDT 2005

What is this acrymount you coverslip with? It sounds like a waterbased 
mounting medium meant for applications, where you can't dehydrate and clear 
with xylene. Do you dehydrate and clear your H&E before mounting?


Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Habitzruther, Michael" <Michael.Habitzruther <@t> RoswellPark.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: "Demant, Peter" <Peter.Demant <@t> RoswellPark.org>
Sent: Tuesday, July 19, 2005 10:56 AM
Subject: [Histonet] Problem with Eosin Y staining

Our laboratory is having a problem with the staining of mouse tumor samples 
using hemotoxylin and Eosin Y (H&E Stain).  Everything was going fine until 
recently.  The specific problem we are having is after the cover-slip is put 
on and the acrymount dries, the Eosin Y stain seems to be bleeding out, 
leaving only the hemotoxylin staining.  A histologist from the institute 
suggested it may have something to do with the relative humidity in our lab, 
and therefore allowing the slides to set under a flow-hood may help.  Any 
other suggestions or protocol modifications would be greatly appreciated. 
Thank you very much.

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