[Histonet] Problem with Eosin Y staining
Michael.Habitzruther <@t> RoswellPark.org
Tue Jul 19 09:56:39 CDT 2005
Our laboratory is having a problem with the staining of mouse tumor samples using hemotoxylin and Eosin Y (H&E Stain). Everything was going fine until recently. The specific problem we are having is after the cover-slip is put on and the acrymount dries, the Eosin Y stain seems to be bleeding out, leaving only the hemotoxylin staining. A histologist from the institute suggested it may have something to do with the relative humidity in our lab, and therefore allowing the slides to set under a flow-hood may help. Any other suggestions or protocol modifications would be greatly appreciated. Thank you very much.
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