[Histonet] Problem with Eosin Y staining

[Habitzruther, Michael]

Our laboratory is having a problem with the staining of mouse tumor samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going fine until recently.  The specific problem we are having is after the cover-slip is put on and the acrymount dries, the Eosin Y stain seems to be bleeding out, leaving only the hemotoxylin staining.  A histologist from the institute suggested it may have something to do with the relative humidity in our lab, and therefore allowing the slides to set under a flow-hood may help.  Any other suggestions or protocol modifications would be greatly appreciated.  Thank you very much.


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