[Histonet] Problem with Eosin Y staining

Habitzruther, Michael Michael.Habitzruther <@t> RoswellPark.org
Tue Jul 19 09:56:39 CDT 2005

Our laboratory is having a problem with the staining of mouse tumor samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going fine until recently.  The specific problem we are having is after the cover-slip is put on and the acrymount dries, the Eosin Y stain seems to be bleeding out, leaving only the hemotoxylin staining.  A histologist from the institute suggested it may have something to do with the relative humidity in our lab, and therefore allowing the slides to set under a flow-hood may help.  Any other suggestions or protocol modifications would be greatly appreciated.  Thank you very much.

This email message may contain legally privileged and/or confidential information.  If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited.  If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

More information about the Histonet mailing list