[Histonet] culture on coverslips troubles

[LaCinda Burchell]

Greetings Histonetters:
  I'm being asked to give advice about how to keep cultured cells on their cover slips during IHC for Confocal Microscopy.  Is there a specific technique for washing the cover slips without losing cells?  Should the cover slips be "air-dried" at some point?  What fixative is best for confocal microscopy?  Are there positively charged cover slips out there?  Last question: Does anyone know of a list server for culture people?   Marketing people please feel free to respond to me regarding these questions if you think you can be of service.   I thank you all, and I send thanks for my questioning friend.

LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave.  CSC  K4/913
Madison, Wisconsin  53792

Phone: 608-262-3518
FAX:     608-263-3746