[Histonet] Need help from Ventana BenmchMark users

[GUTIERREZ, JUAN]

Hello Sharon,
We use Ventana's TdT (SEN28)and Dakocytomation's Hepatocyte antibodies.  For the TdT we have found that using the Enhanced V-Red chromogen works much better than the i-View DAB (less background). 
The protocols are:
TdT
Standard CC1
Option 1 for 8 minutes (Fc receptor block from INNOVEX Biosciences 1-800-622-7808)
Antibody for 16 min (32 min if using i-View)
Amplify
A/B block
Counterstain

Hepatocyte 
Standard CC1
Antibody for 32 min at a 1:25-1:50 dilution
A/B block 
Counterstain

These are done on a regular Benchmark, not the LT or XT.  Good luck with it and I hope this helps.


Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live free speech!


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of E Sharon Shields
Sent: Tuesday, July 12, 2005 3:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need help from Ventana BenmchMark users

Hi all,
I have been asked to develop stain protocols for TdT on bone marrow aspirates and Hepatocyte Specific Antigen on FFPE tissues. Does anyone out there in Histoland doing these two IHC stains on the BenchMark?  Would you be willing  to share your antibody source and stain protocol?
 
Thank you,
Sharon Shields
Bapitist Hospital of East Tennessee
Knoxville, TN
phone 865 549-4351
fax 865 632-5316


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