[Histonet] Need help from Ventana BenmchMark users
juan.gutierrez <@t> christushealth.org
Wed Jul 13 05:15:31 CDT 2005
We use Ventana's TdT (SEN28)and Dakocytomation's Hepatocyte antibodies. For the TdT we have found that using the Enhanced V-Red chromogen works much better than the i-View DAB (less background).
The protocols are:
Option 1 for 8 minutes (Fc receptor block from INNOVEX Biosciences 1-800-622-7808)
Antibody for 16 min (32 min if using i-View)
Antibody for 32 min at a 1:25-1:50 dilution
These are done on a regular Benchmark, not the LT or XT. Good luck with it and I hope this helps.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
My opinions are my own and do not reflect those of my employer. Long live free speech!
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of E Sharon Shields
Sent: Tuesday, July 12, 2005 3:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need help from Ventana BenmchMark users
I have been asked to develop stain protocols for TdT on bone marrow aspirates and Hepatocyte Specific Antigen on FFPE tissues. Does anyone out there in Histoland doing these two IHC stains on the BenchMark? Would you be willing to share your antibody source and stain protocol?
Bapitist Hospital of East Tennessee
phone 865 549-4351
fax 865 632-5316
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