[Histonet] Result : HIER - best retrieval with minimum tissue damage
n.cragg <@t> epistem.co.uk
Wed Jul 13 03:20:38 CDT 2005
Thanks for everyone who has suggested gentler heat methods - waterbath heating was successful in retrieving the antigen of interest (PGP9.5 as a marker for cutaneous nerve fibres) in skin, while maintaining dermal integrity. Tried 2 buffers - citrate pH6.0 and EDTA pH8.0, preferred the citrate this time as a little cleaner, but not much between them. Will definitely use this method again as part of the optimisation process for other dermal antigens which require heat retrieval.
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