[Histonet] Result : HIER - best retrieval with minimum tissue damage

Nicola Cragg n.cragg <@t> epistem.co.uk
Wed Jul 13 03:20:38 CDT 2005


Thanks for everyone who has suggested gentler heat methods - waterbath heating was successful in retrieving the antigen of interest (PGP9.5 as a marker for cutaneous nerve fibres) in skin, while maintaining dermal integrity.  Tried 2 buffers - citrate pH6.0 and EDTA pH8.0, preferred the citrate this time as a little cleaner, but not much between them.  Will definitely use this method again as part of the optimisation process for other dermal antigens which require heat retrieval.

Thanks again,

Nicola Cragg
EpiStem Ltd.
Manchester, UK

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