[Histonet] HIER - best retrieval with minimum tissue
Rcartun <@t> harthosp.org
Mon Jul 11 14:29:22 CDT 2005
What fixative do you use? How long do you fix?
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
80 Seymour Street
Hartford, CT 06102
(860) 545-0174 Fax
>>> "Kristopher Kalleberg" <Kristopher.Kalleberg <@t> unilever.com> 07/11/05 03:01PM >>>
I almost exclusively perform IHC on skin and always use the pressure cooker for
HIER. I rarely have any problems or encounter disruptions in the morphology of
the sample. I put the slides in the pressure cooker and when the pressure
reaches the appropriate level I let them cook for 6 minutes. It seems to be a
fail-safe. Hope this helps.
40 Merritt Blvd.
Trumbull CT 06611
203 381 5765
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