[Histonet] HIER - best retrieval with minimum tissue damage?

Kristopher Kalleberg Kristopher.Kalleberg <@t> unilever.com
Mon Jul 11 14:01:39 CDT 2005

I almost exclusively perform IHC on skin and always use the pressure cooker for
HIER.  I rarely have any problems or encounter disruptions in the morphology of
the sample.  I put the slides in the pressure cooker and when the pressure
reaches the appropriate level I let them cook for 6 minutes.  It seems to be a
fail-safe.  Hope this helps.

Kris Kalleberg
Unilever R&D
40 Merritt Blvd.
Trumbull CT 06611
203 381 5765

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