[Histonet] University of Pittsburgh

Stephen Peters M.D. petepath <@t> yahoo.com
Wed Jul 6 08:56:27 CDT 2005


Are the nuclei totally missing? or are there holes in the nuclei? If there are holes in the nuclei 
I believe this is one of the manifestations of freeze artefact which becomes very obvious 
in sections in the under 3 micra range. Even though a cryostat is set for 5 micra it is very
 common for the first few sections to be different thickness, commonly a thick one followed 
by a thin one. 
I show pics of what I believe are nuclear ice crystals and the effect of section thickness on
 this page of my frozen section tutorial:  http://pathologyinnovations.com/new_page_3.htm.
 
If the nuclei are falling out, I have no answer.
 
Stephen


Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 
www.pathologyinnovations.com 







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