[Histonet] Impress

[Van Eyck, Deb]

Dear-histonetters-----I have also wondered about the Impress Kit----Amos and
to otheres who tried it---was it pretty interchangeable- time wise with the
Envision , or Envision + also what about antibody dilutions? same strength?


Another immuno question for you all?  is anyone doing Toxoplasmosis
staining?   What antibody are you using? What protocol?  Thanks much-Deb Van
Eyck



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> Subject:	Histonet Digest, Vol 20, Issue 3
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>    1. Re: Histonet Digest, Vol 19, Issue 43 (Amos Brooks)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Sat, 02 Jul 2005 16:19:45 -0400
> From: Amos Brooks <amosbrooks <@t> earthlink.net>
> Subject: [Histonet] Re: Histonet Digest, Vol 19, Issue 43
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <42C6F6E1.2010501 <@t> earthlink.net>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> 
> Carla,
> 
> 	We use both depending on the antibody. Mostly we use Envision, but
> we tried the Impress on some of the antibodies that don't label very
> intensely and did see improvement. If you compare the results of a CD2,
> CD4, CD7 or CD8 odds are you'll see some improvement. Not all the
> antibodies showed marked improvement and in some cases envision was better
> so we use Envision on most but the success we've had with Impress made it
> worth switching some of the procedures. Vector might give you a sample if
> you ask nicely. Give it a whirl and see if you like it.
> 
> Best of luck,
> Amos
> 
> 
> 
> Message: 17
> Date: Thu, 30 Jun 2005 07:34:30 -0700
> From: Carla M Conway <cmconway <@t> usgs.gov>
> Subject: [Histonet] Dako EnVision G2 or Vector ImmPRESS comments
> 	wanted  
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OFF6D9EC48.FF33E03E-ON88257030.004D25F6-88257030.00501497 <@t> usgs.gov>
> Content-Type: text/plain; charset=US-ASCII
> 
> Hello all,
> 
> I would like any comments (pro and con) regarding Dako's EnVision G2/AP
> system or Vector's ImmPRESS kit. We are currently using EnVision + , but
> are wondering if these new systems could be even better (more sensitive).
> 
> Thanks for your help,
> 
> Carla Conway
> 
> Western Fisheries Research Center
> 6505 NE 65th St
> Seattle, WA 98115
> ph: 206-526-6282 ext. 242
> fax: 206-526-6654
> cmconway <@t> usgs.gov
> 
> 
> 
> 
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> End of Histonet Digest, Vol 20, Issue 3
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