[Histonet] reversible tissue adhesion to slides

[Patsy Ruegg]

I release super glued tissue using a single edge razor blade.
Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Philip Oshel
Sent: Tuesday, January 11, 2005 7:33 AM
To: Histonet <@t> Pathology.swmed.edu
Subject: Re: [Histonet] reversible tissue adhesion to slides

Janis,

How about attaching the part you *don't* want? That way the dissection will
also release the desired tissue. Use a minute (no! 
smaller! still smaller ... ) drop of superglue to hold the tissue to the
slide -- the same glue used to glue fresh tissue to the mounting plate of a
vibrating microtome.

Phil

>Dear colleagues,
>
>We are looking for a method to very firmly attach unfixed larval 
>Drosophila ventral ganglia to a glass slide so we can slice off one 
>surface of the ganglion (to remove neurons that we don't want; it's a 
>long story; e-mail me if you want to know), to be followed by 
>*releasing the attached tissue* from the slide and preparing primary 
>neuronal cultures.  The tissue needs to be kept alive and happy under 
>saline.  Polylysine doesn't hold tight enough for the slicing step 
>(which we do with a fine blade).  Possibilities that have come to mind 
>are using more adhesive slides (e.g., coated with aminosilane?), using 
>some sort of double-stick tape, or a releasable glue (e.g., something 
>like vetbond but that can be made to release?).  Obviously part of the 
>difficulty is to release the tissue
>without destroying it.   Does anyone have a suggestion?  thanks!
>
>Janis Weeks
>
>--=[|]=-- --=[|]=-- --=[|]=-- --=[|]=-- --=[|]=-- Janis C. Weeks, 
>Professor of Biology weeks <@t> uoneuro.uoregon.edu 
>http://www.neuro.uoregon.edu/faculty/weeks.html
>Institute of Neuroscience
>1254 University of Oregon
>Eugene, OR  97403-1254
>voice (541)346-4517, FAX (541)346-4548
>--=[|]=-- --=[|]=-- --=[|]=-- --=[|]=-- --=[|]=--
>
>
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

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