[Histonet] Myelin basic Protein in mouse

Koelling, Ray koellinr <@t> amgen.com
Fri Jan 7 14:43:46 CST 2005


Cynthia,
for a couple of years we've run rat-antiMBP from Serotec (MCA408) on mouse
brain fixed 24 hours in NBF.  At 1:1000-1:2000 overnight at 4 degrees with
no retrieval what-so-ever. Then biotinylated rabbit anti rat and so forth.
Ray
Raymond Koelling
Research Scientist, Pathology
Amgen Corp.
Seattle, WA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Favara,
Cynthia (NIH/NIAID)
Sent: Friday, January 07, 2005 11:21 AM
To: Histonet
Subject: [Histonet] Myelin basic Protein in mouse


I have run a quick search in the archives and found nothing. Anyone with
experience with Myelin Basic Protein  immunohistochemistry in FFPE murine
tissue?

Thanks,
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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