[Histonet] GFP

Carol Bobrowitz carolb <@t> mail.phys.mcw.edu
Fri Feb 25 14:21:06 CST 2005

Has anyone worked with GFP tissue?

I have found GFP is surviving frozen sections, acetone and 100% alcohol. 

I am wondering about FFPE tissue.  I'm thinking the heat during processing
would destroy the GFP.

Does formalin fixation effect the GFP?

Is it possible to fix the tissue in an alcoholic fixative and manually hand
process at room temperature and infiltrate into plastic?   Would the plastic
medium destroy the GFP?

What about celloidin embedded tissue?  This is not my choice

Any information would be helpful.

I thank everyone on the histonet that has in the past and future helped me.
I really appreciate your help.


Carol Ann Bobrowitz
Histology Laboratory
Department of Physiology - Room 541
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, Wisconsin   53226
FAX 414-456-6546
cbobrowi <@t> mcw.edu

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