[Histonet] lectins

[Paul Webster]

Jeurgen Roth and John Lucocq did lots of studies conjugating lectins to
colloidal gold particles about 20 years ago. I think they finally concluded
that it was more efficient to use sequential labeling protocols with
secondary labeling reagents for detecting lectins. That is, you react the
lectin with the section, bind an anti-lectin antibody to the lectin and then
visualize with a gold-conjugated secondary antibody (or protein a gold).

One method they described used fetuin-gold to detect lectin bound to the
section (Light and electron microscopic demonstration of sialic acid
residues with the lectin from Limax flavus: a cytochemical affinity
technique with the use of fetuin-gold complexes. J Histochem Cytochem. 1984
Nov;32(11):1167-76.).

Paul Webster.



 
Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922
Phone: 213 273 8026
Fax: 213 13 739
E-mail: pwebster <@t> hei.org






On 2/24/05 6:06 AM, "Margaret Horne" <mhorne <@t> upei.ca> wrote:

> Hello Everyone , 
>       Has anyone out there conjugated gold colloid to lectins?
> Some gold conjugated lectins I can buy but others I may have to
> make.
> 
>     Thanks , 
> Margaret
> 
> 
> 
> 
> Margaret Horne ,
> Histology Teaching Assistant,
> Dept. of B.SC.,
> Atlantic Veterinary College, U.P.E.I.,
> 550 University Ave., Charlottetown,
> P.E.I.,  C1A 4P3 
> Canada
> 
> 
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