[Histonet] stain for calcium oxalate on paraffin

Hermina Borgerink hborgeri <@t> wfubmc.edu
Tue Feb 22 14:18:06 CST 2005

Pizzolato's stain is generally used for the demonstration of calcium
oxalate crystals in paraffin.  The complete technique is in Theory and
Practice of Histotechnology by Sheehan and Hrapchak, p. 228.

1.  Hydrate slides
2.  Mix equal volumes of 30% hydrogen peroxide and 5% silver nitrate.  
3.  Flood slides with the mixture and expose to a 60-watt
tungsten-filament electric lamp or a 25-watt fluorescent bulb.  The
light should be approximately 15 cm above the sections.  Exposure varies
from 15 to 30 minutes.  If excessive bubbling is observed re place the
regent mixture with a fresh one.
4.  Rinse thoroughly in distilled or deionized water.
5.  Counterstain with 0.1% safranin in 1% acetic acid for 2-3 minutes.
6.  Rinse in distilled or deionized water, dehydrate, clear and

Calcium oxalate - black
Nuclei - red

Note:  Calcium fluoride and calcium and barium sulfates do not react by
this method.


Hermina M. Borgerink, BA, HTL(ASCP)QIHC
Wake Forest University Health Sciences
Department of Pathology
Medical Center Blvd.
Winston-Salem, NC 27157
Tel. (336) 716-1538
Fax. (336) 716-1515
e-mail hborgeri <@t> wfubmc.edu 
"Treat a man as he appears to be, and you make him worse.  But treat a
man as if he already were what he potentially could be, and you make him
what he should be."  (Goethe)
This electronic message, including any attachments, is confidential and
proprietary and is solely for the intended recipient.  If you are not
the intended recipient, this message was sent to you in error and you
are hereby advised that any review, disclosure, copying, distribution or
use of this message, or any of the information included therein, is
unauthorized and strictly prohibited.  If you have received this
electronic transmission in error, please immediately notify the sender
by reply and permanently delete all copies of this message and its
attachments.  Thank you. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barb
Sent: Tuesday, February 22, 2005 2:20 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] stain for calcium oxalate on paraffin

We are looking for a stain that we can use on a paraffin embedded renal
biopsy to determine oxalate crystals.  Does anyone know of any??

Confidentiality Notice: This e-mail message, including any attachments,
for the sole use of the intended recipient(s) and may contain
and privileged information. Any unauthorized review, use, disclosure, or
distribution is prohibited. If you are not the intended recipient,
contact the sender by reply e-mail and destroy all copies of the

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list