[Histonet] stain for calcium oxalate on paraffin

[Hermina Borgerink]

Pizzolato's stain is generally used for the demonstration of calcium
oxalate crystals in paraffin.  The complete technique is in Theory and
Practice of Histotechnology by Sheehan and Hrapchak, p. 228.

1.  Hydrate slides
2.  Mix equal volumes of 30% hydrogen peroxide and 5% silver nitrate.  
3.  Flood slides with the mixture and expose to a 60-watt
tungsten-filament electric lamp or a 25-watt fluorescent bulb.  The
light should be approximately 15 cm above the sections.  Exposure varies
from 15 to 30 minutes.  If excessive bubbling is observed re place the
regent mixture with a fresh one.
4.  Rinse thoroughly in distilled or deionized water.
5.  Counterstain with 0.1% safranin in 1% acetic acid for 2-3 minutes.
6.  Rinse in distilled or deionized water, dehydrate, clear and
coverslip. 

Calcium oxalate - black
Nuclei - red

Note:  Calcium fluoride and calcium and barium sulfates do not react by
this method.

Hermina

Hermina M. Borgerink, BA, HTL(ASCP)QIHC
Wake Forest University Health Sciences
Department of Pathology
Medical Center Blvd.
Winston-Salem, NC 27157
Tel. (336) 716-1538
Fax. (336) 716-1515
e-mail hborgeri <@t> wfubmc.edu 
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barb
Richmond
Sent: Tuesday, February 22, 2005 2:20 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] stain for calcium oxalate on paraffin


We are looking for a stain that we can use on a paraffin embedded renal
biopsy to determine oxalate crystals.  Does anyone know of any??

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