[Histonet] alk phos, again
Rachael_Emerson <@t> URMC.Rochester.edu
Tue Feb 22 13:12:56 CST 2005
Other there other methods to block endogenous alkaline phosphatase?
I am using mouse embryos, frozen sectioned. I've tried:
levamisole at varying concentrations: still background
heated PBS at varying times: too strong, blocked my signal
15% acetic acid at 15": too strong, blocked my signal
5% acetic acid at 15": still background
usually I did a combo of levamisole and another treatment.
I appreciate any thoughts.......
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