[Histonet] Negative Rabbit Contrl

[Kelly D Mcqueeney]

Louis,
I have seen the same problem with rabbit IgG. I always use the rabbit 
IgG from Vector or Jackson as a control. Once you receive the IgG, 
immediately aliquot (5ul if possible) and store at -20 or -80C. Once you 
thaw and use an aliquot, immediately throw it away. Do not leave at 4C 
or re-freeze. I only get negative results with a fresh, frozen aliquot 
used once.

Good luck,
Kelly

Louise Strande wrote:

>Does anyone have a source for a good normal rabbit IgG to use for negative control immunohistochemical applications?   I have human tissue or human cytospins and occassionally my primary antibody is grown in rabbit.  In order to run a non immunized rabbit negative control,   I have purchased normal rabbit IgG from DAKO, Zymed, Santa Cruz, Lab Visions.  Every one I try gives positive staining on the negative slide.  I have done the appropriate tests and the staining is not coming from streptaviden, or the link anibody, but from the rabbit IgG itself.  I do not have this problem with Mouse negative control IgG or goat negative control IgG.   Any suggestions, other than not buying any antibodies rasied in rabbit?
>
>Louise Strande, MS
>Cooper University Hospital
>Department of Surgery
>Division of Surgical Research
>Phone : (856) 757-7827
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