[Histonet] Negative Rabbit Contrl

Louise Strande strande <@t> umdnj.edu
Mon Feb 21 15:01:22 CST 2005

Does anyone have a source for a good normal rabbit IgG to use for negative control immunohistochemical applications?   I have human tissue or human cytospins and occassionally my primary antibody is grown in rabbit.  In order to run a non immunized rabbit negative control,   I have purchased normal rabbit IgG from DAKO, Zymed, Santa Cruz, Lab Visions.  Every one I try gives positive staining on the negative slide.  I have done the appropriate tests and the staining is not coming from streptaviden, or the link anibody, but from the rabbit IgG itself.  I do not have this problem with Mouse negative control IgG or goat negative control IgG.   Any suggestions, other than not buying any antibodies rasied in rabbit?

Louise Strande, MS
Cooper University Hospital
Department of Surgery
Division of Surgical Research
Phone : (856) 757-7827

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