[Histonet] re-embedding blocks

[John Kiernan]

I don't know about "generally". When I do this
(which isn't often) I use the same temperature as 
for infiltration, which in my lab is about 60C for 
a 58C wax. It works. Why do otherwise? 

With regard to "a temperature that is generally 
considered too high either for the paraffin or 
for the tissue", you can expect a variety of
replies because this is controversial. A popular
notion is that too-hot makes the tissue unduly
hard to cut. I don't agree, but that's another
story, and there's plenty of HISTOMYTHOLOGY in
this area. 

John Kiernan
Dept of anatomy & Cell Biology
University of Western Ontario
London, Canada.
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Bartlett, Jeanine" wrote:
> 
> Hi everybody:
> 
> Just curious as to what oven temperature is generally used for melting
> down paraffin blocks for re-embedding.  Is there a temperature that is
> generally considered too high either for the paraffin or for the tissue?
> 
> Thanks!
> Jeanine Bartlett, HT(ASCP)
> Centers for Disease Control and Prevention
> Infectious Disease Pathology Activity
> 1600 Clifton Road, MS/G-32
> Atlanta, GA 30333
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